Welcome Guest ( Log In | Register )

Reply to this topicStart new topic
> Question about stimulator not working in whole cell patch clamp
post Jul 21, 2011, 11:45 AM
Post #1


Group: Basic Member
Posts: 1
Joined: Jul 21, 2011
Member No.: 33465

Hi Guys,

I have a prob and it'd be great if somebody could give me an idea how to solve it or just throw an idea!!

So I've been doing voltage patch clamping, whole cell recording. I can record beautiful mIPSC/EPSC. Now I want to record evoked IPSC so I'm using a stimulator injecting current. I saw the artifact and it goes up and down to the intensity of my stimulation. I checked the connectivity of the system and it's all fine. I put the stimulator fairly close to my neuron and it should work. But I just can't see a response IPSC/EPSC. When I put only the stimulator, patch pipette and ground in the bath, I also only see the artifact, no current.

Anybody knows what's happening here possily, please?

Thank you!

User is offlineProfile CardPM
Go to the top of the page
+Quote Post

Reply to this topicStart new topic
1 User(s) are reading this topic (1 Guests and 0 Anonymous Users)
0 Members:


Lo-Fi Version Time is now: 15th September 2019 - 04:48 AM

Home     |     About     |    Research     |    Forum     |    Feedback  

Copyright BrainMeta. All rights reserved.
Terms of Use  |  Last Modified Tue Jan 17 2006 12:39 am

BrainMeta is supported by:

The Neurological Foundation & CerebralHealth.com

BrainMeta is enhanced by:

UVISI: Universal Virtual Intelligence Singularity Infinity